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Institute of Ophthalmology, University College London

Dr. Li Li Chen

Research Fellow

The duties of my work are:

Assisting the group’s leading scientist to investigate the potential of the human embryonic stem cells to acquire a retinal cell fate both in vitro and in vivo and to develop efficient cell-based treatments for currently untreatable Age-related Macular Degeneration (AMD).

Assessment of the visual improvement after transplantation by doing the animal behaviour analysis (i.e. acuity & contrast sensitivity).

Immunohistochemistry analysis on transplanted tissues to confirm the disease and the retinal repair on animal models.

Also involved in everyday running & management of the laboratory (i.e. stocks of antibody & animal information database).  

Key achievements

Completed a Ph.D. degree at the Biochemistry Department, Imperial College (London) studying Molecular Biology.

Setting up the sera, lymphocytes, genomic DNA bank and the database for AMD project study in the UK. Taking part in the management of the ophthalmic genetic database.

Sub-cloning, purification & protein structure/functional characterisation of seven mutant HSODs (Human CuZn Superoxide Dismutase) causing FALS (Familial Amyotrophic Lateral Sclerosis).

Raised a monoclonal antibody for detecting BCL-10 nuclear localization in MALT (Mucosa-Associated Lymphoid Tissue) lymphoma.

Co-run a final year undergraduate practical & the project student from medical school.

Experience with a wild range of laboratory analytical techniques & laboratory management.

Experience with use the OptoMotry 1.7.0 virtual opto-kinetic system (CerebralMechanics Inc.) for laboratory rodents.

Key publications

  • Peter J. Coffey, Carlos Gias, Caroline J. McDermott, Peter Lundh, Matthew C. Pickering, Charanjit Sethi, Alan Bird, Fred W. Fitzke, Annelie Maass, Li Li Chen, Graham E. Holder, Philip J. Luthert, Thomas E. Salt, Stephen E. Moss, and John Greenwood. Complement factor H-deficiency in aged mice causes retinal abnormalities and visual dysfunction. PNAS, October 16, 2007, 104(42): 16651-16656.
  • M Michaelides, L L Chen, M A Brantley Jr,  J L Andorf, E M Isaak, S A Jenkins, G E Holder, A C Bird, E M Stone, A R Webster.  ABCA4 Mutations and Discordant ABCA4 Alleles in Patients and Siblings with Bull's-Eye Maculopathy. Manuscript accepted for Online First by Br J Ophthalmol 2007;91:1650-1655, doi: 10.1136/bjo.2007.118356

  • N.D. Ebenezer, C. Patel, S Hariprasad, L.L.Chen, R.J. Patel, S.S. Bhattacharya, A.R. Webster, R.C. Allen. Clinical and molecular characterization of a family with autosomal recessive cornea plana.. Arch Ophthalmol. 2005 Sep; 123(9):1248-53.
  • H. Ye, A. Dogan, L. Karran, T.G. Willis, L.L.Chen, I. Wlodarska, M.J. Dyer, P.G. Isaacson, M.Q. Du. (2000) BCL10 expression in normal and neoplastic lymphoid tissue:nuclear localization in MALT lymphoma. Am J Pathol 157(4):1147-54.
  • S.I. Liochev, L.L.Chen, R.A. Hallewell andI. Fridovich. (1998) The FALS-associated amino acid substitutions, E100G, G93A and G93R, do not influence the rate of inactivation of Cu, ZnSOD by H2O2. Arch. Biochem. Biophys. 352, 237-239.